WHO

 WHO 1999(4th)

ParameterReference value
Ejaculate volume (ml)≧ 2
pH≧ 7.2
Sperm concentration (million/ml)

≧ 2

Total motility (PR+NP,%)≧ 50%
Progressive motility (%)≧ 25%
Normal morphology (%)                      ≧ 30%
Leukocyte number (million/ml)< 1

 WHO 2010 (5th)

ParameterLowr reference limit
Semen volume (ml)1.5 (1.4-1.7)
Total sperm number(ml/ejaculate)39 (33-46)
Sperm concentration (106per ml)15 (12-16)
Total motility (PR+NP,%)40 (38-42)
Progressive motility (PR,%)32 (31-34)
Vitality (live spermatozoa,%)58 (55-63)
Sperm morphology (normal forms,%)4 (3.0-4.0)
Peroxidase-positive leukocyte number (million/ml)< 1
Other consensus threshold values
pH≧ 7.2
Seminal fructose (μmol/ejaculate)≧ 13
Seminal neutral glucosidase (mU/ejaculate)≧ 20






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